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| DNA Genotyping of Oral Epithelial Cells by Laser Capure Microdissection |
| 2008-5-24 |
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GU Li- hua, ZHANG Cheng, CHEN Lian- kang, ZHEN Hui- fen, CHENG Li, ZHOU Huai- gu
( Shanghai Institute of Forensic Science, Shanghai, 200083, china)
Abstract: objective The STR genotypping of trace oral epithelial cells which are microdissected by laser capure microdissection system (LCM) is explored. Methods The oral epithelial cells are microdissected using a low- power infrared laser by VERITAS Microdissection Instrument. STR loci of Profiler Plus are
detected by multiplex PCR procesures. Results DNA genotyping of 7- 8 oral epithelial cells are succeeded, and DNA genotyping of 3- 4 oral epithelial cells are failed. Conclusion It is viable in genotyping of trace oral epithelial cells by Laser Capure Microdissection as a new technology of seperating single cell.
Key words: laser capure microdissection(LCM); oral epithelial cells; STR- DNA typing |
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